HIF1α regulates PS expression in the mouse liver. (A) Representative immunoblots showing relative PS and HIF1α protein levels in livers from HIF1α liver-specific knockout mice, control mice, and liver-specific HIF1α dPA mice. Each blot contained samples from 2 mice belonging to each category. The reported data are representative of 3 separate immunoblots, and the blot densities are quantified in supplemental Table 1. (B) Relative PS mRNA levels in the livers of control mice, HIF1α liver-specific knockout (KO) mice, and liver-specific HIF1α dPA mice (n = 5 per group). (C) Representative immunoblots assessing relative amounts of PS in plasmas from HIF1α liver-specific knockout mice, liver-specific HIF1α dPA mice, and control mice. (D) The graph shows relative thrombin generation by the plasmas from HIF1α liver-specific knockout mice, control mice, and liver-specific HIF1α dPA mice. (E) The graph shows thrombin generation by plasma from liver-specific HIFα dPA mice that was inhibited by addition of exogenous PS. (F) The graph shows relative thrombin generation from control mouse plasma following addition of anti-PS antibody or exogenous PS. (G) Thrombin generation by plasma from HIF1α liver-specific knockout mice in the presence of PS or anti-PS antibody. (H) Model depicting stabilization of HIF1α in mouse liver suppresses PS expression. ****P < .0001.