CD8⁺Tregs and Tcons have similar T-cell receptor repertoire diversity. Lethally irradiated (900 cGy) Balb/c mice were transplanted with B6 Rag-1 BM (5 × 10⁶) and flow-sorted CD4⁺ Foxp3⁻ (0.4 × 10⁶) and CD8⁺ Foxp3⁻ (0.2 × 10⁶) T cells from B6 Foxp3^EGFP animals. Mice were euthanized on day 7 posttransplantation, and donor-derived CD4⁺ Foxp3⁺, CD8⁺ Foxp3⁺, CD4⁺ Foxp3⁻, and CD8⁺ Foxp3⁻ T cells were sorted from the spleens and livers of recipients. (A) Shannon entropy of CD4⁺ Foxp3⁺ (n = 4), CD4⁺ Foxp3⁻ (n = 6), CD8⁺ Foxp3⁺ (n = 6), and CD8⁺ Foxp3⁻ (n = 6) T-cell populations. Error bars represent standard error of the mean. (B) Morsita Horn similarity index of CD4⁺ Tregs and Tcons (n = 4) and CD8⁺ Treg and Tcons (n = 6). Colored circles denote individual mice. (C) Heat map showing Morsita Horn diversity overlap of TCRβ clonotype expression in CD4⁺ and CD8⁺ Treg and Tcon populations from individual mice. (D) Lethally irradiated (900 cGy) Balb.B mice were transplanted with B6 Foxp3^EGFP BM (5 × 10⁶) and spleen cells (adjusted to yield a dose of 20 × 10⁶ αβ T cells). Animals were euthanized on day 7 posttransplantation, and cells were harvested from the liver and spleen. Representative contour plot depicting CD8⁺ H60⁺ Foxp3⁺ and CD8⁺ H60⁺ Foxp3⁻ T cells. (E) Shannon entropy of CD8⁺ H60⁺ Treg (n = 2) and Tcon (n = 2) populations. Error bars represent standard error of the mean. (F) Heat map showing Morsita Horn diversity overlap of TCRβ clonotype expression in CD8⁺ H60⁺ Foxp3⁺ and Foxp3⁻ T cells from individual mice. *P < .05; **P < .01.