Gene expression differences between CD8⁺and CD4⁺Tregs. (A) Waterfall plot of genes with significantly increased expression (red bars) or decreased expression (blue bars) in in vivo-induced CD8⁺ Tregs relative to in vivo-induced CD4⁺ Tregs, but not in CD8⁺ Tcons relative to CD4⁺ Tcons. (B) Gene pathways that are differentially expressed in in vivo-induced CD8⁺ versus in vivo-induced CD4⁺ Tregs by Gene Set Enrichment analysis. (Ci-ii) Gene pathways that are differentially expressed in in vivo-induced CD8⁺ vs in vivo-induced CD4⁺ Tregs by Ingenuity Pathway analysis. (D) Expression of selected genes coding for antiapoptotic and proapoptotic proteins in in vivo-induced CD8⁺ Treg, CD8⁺ Tcon, CD4⁺ Treg, and CD4⁺ Tcon cells. **P < .01.