Fig. 1.
Fig. 1. Autoradiograms of BM biopsy specimens from HAM/TSP patients and normal, uninfected controls following PCR/ISH and ISH (hematoxylin and eosin counterstain, which stains nuclei purple and cytoplasm pink) (all photomicrographs are original magnification ×400). (a) PCR/ISH of HTLV-I-tax DNA in a BM autopsy specimen from patient 1. Extensive HTLV-I-tax DNA signal (brightfield microscopy, silver grains appear black). (b) PCR/ISH of HTLV-I-tax DNA in a BM biopsy specimen from patient 2. Extensive HTLV-I-tax DNA signal present (combined epiflourescence and transillumination, silver grains appear green above purple, hematoxylin-stained cells); however, there is minimal signal in endothelial cells surrounding blood vessels (arrow). (c) PCR/ISH of HTLV-I-tax DNA in a BM biopsy specimen from patient 3. Extensive HTLV-I-tax DNA signal present. (d) PCR/ISH of HTLV-I-tax DNA from an HAM/TSP patient, showing minimal signal around a megakaryocyte (arrow). (e) PCR/ISH of HTLV-I-tax DNA from an HAM/TSP patient, showing minimal signal of mature red blood cells surrounding a positive cell (arrow). (f ) PCR/ISH using HTLV-I-tax DNA specific primers plus Taq polymerase, after detection of the HTLV-I-tax–amplified product with a heterologous probe. There is no positive signal. (g) PCR/ISH of HTLV-I-tax DNA in a BM biopsy specimen of an uninfected, normal patient. There are no positive cells. (h and i) PCR/ISH of HTLV-I-tax DNA of the BM biopsy of patient 1, excluding Taq polymerase (h) or HTLV-I-tax–specific primers (i) from the PCR cocktail, and detection of the amplified product with the HTLV-I-tax probe. No positive cells. (j) Modified Giemsa (Lennert) stain of a representative field of BM biopsy specimen from an HAM/TSP patient showing normal hematologic morphology. (k) ISH for HTLV-I-tax DNA. There are no positive cells and no nonspecific probe binding to eosinophils (arrow). (l) ISH for HTLV-I-tax RNA in a BM biopsy specimen of an HAM/TSP patient. A single positive cell (arrow) is present. (Inset) Darkfield microscopy of same cell showing white silver grains above cell nucleus.

Autoradiograms of BM biopsy specimens from HAM/TSP patients and normal, uninfected controls following PCR/ISH and ISH (hematoxylin and eosin counterstain, which stains nuclei purple and cytoplasm pink) (all photomicrographs are original magnification ×400). (a) PCR/ISH of HTLV-I-tax DNA in a BM autopsy specimen from patient 1. Extensive HTLV-I-tax DNA signal (brightfield microscopy, silver grains appear black). (b) PCR/ISH of HTLV-I-tax DNA in a BM biopsy specimen from patient 2. Extensive HTLV-I-tax DNA signal present (combined epiflourescence and transillumination, silver grains appear green above purple, hematoxylin-stained cells); however, there is minimal signal in endothelial cells surrounding blood vessels (arrow). (c) PCR/ISH of HTLV-I-tax DNA in a BM biopsy specimen from patient 3. Extensive HTLV-I-tax DNA signal present. (d) PCR/ISH of HTLV-I-tax DNA from an HAM/TSP patient, showing minimal signal around a megakaryocyte (arrow). (e) PCR/ISH of HTLV-I-tax DNA from an HAM/TSP patient, showing minimal signal of mature red blood cells surrounding a positive cell (arrow). (f ) PCR/ISH using HTLV-I-tax DNA specific primers plus Taq polymerase, after detection of the HTLV-I-tax–amplified product with a heterologous probe. There is no positive signal. (g) PCR/ISH of HTLV-I-tax DNA in a BM biopsy specimen of an uninfected, normal patient. There are no positive cells. (h and i) PCR/ISH of HTLV-I-tax DNA of the BM biopsy of patient 1, excluding Taq polymerase (h) or HTLV-I-tax–specific primers (i) from the PCR cocktail, and detection of the amplified product with the HTLV-I-tax probe. No positive cells. (j) Modified Giemsa (Lennert) stain of a representative field of BM biopsy specimen from an HAM/TSP patient showing normal hematologic morphology. (k) ISH for HTLV-I-tax DNA. There are no positive cells and no nonspecific probe binding to eosinophils (arrow). (l) ISH for HTLV-I-tax RNA in a BM biopsy specimen of an HAM/TSP patient. A single positive cell (arrow) is present. (Inset) Darkfield microscopy of same cell showing white silver grains above cell nucleus.

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