Expression profile of KMUKO myeloid progenitors resembles AML. Bone marrow progenitors were isolated from 7-9-week-old KM and KMUKO mice and analyzed by RNA sequencing. (A) Volcano plot analysis of all transcripts in KMUKO vs KM myeloid progenitors. (B) Top 10 pathways affected in KMUKO cells compared with KM cells. (C) mRNA expression of myeloperoxidase (MPO), elastase, cathepsin G, and proteinase 3 in bone marrow progenitors from the indicated mice. GSEA showing that genes upregulated in KMUKO progenitors are significantly correlated with genes associated with embryonic stem cells (D) and leukemia stem cells (E). Targets of the Myc oncogene were highly enriched in KMUKO progenitors (F), whereas PU.1 target genes were inversely correlated (G). (H) Heat map showing PU.1 target genes from analysis in (G).