(A) Fibrin network formed under static conditions (Fb-static). Tissue factor–rich ECM-coated coverslips were incubated with PPP in a 6-well plate (10 minutes at 37°C). Arrows indicate neutrophils that have adhered after a perfusion with purified resting neutrophils. (B) Fibrin network formed at low shear. ECM-coated cover slips were perfused with PPP from heparinized blood at shear stress 20 mPa (10 minutes at 37°C). (C) Fibrin network formed at high shear stress. ECM-coated cover slips were perfused with PPP from heparinized blood at a shear stress of 80 mPa (10 minutes at 37°C). (D) Fibrin network with platelets (Fb + platelets). ECM-coated cover slips were perfused twice: first with PPP from heparinized blood at a shear stress of 20 mPa (10 minutes at 37°C), followed by perfusion with citrated whole blood for 7 minutes at a shear rate of 1,600 s−1 (ie, a shear stress of ∼7 Pa). As a result, platelets covered 30% to 40% of the fibrin network (arrows).
