RT-PCR analysis of the expression of IL-15R α (upper panel) and control β-actin (lower panel) in highly purified GL from 6 LDGL patients. Lane 1, molecular weight marker (Molecular weight VI–digested); lane 2, negative control (sample without cDNA); lane 3, control PBMC stimulated for 12 hours with PHA (5 μg/mL); lanes 4 to 6, 3 CD3⁺ LDGL cases (cases no. 1, 3, and 9 in Table 1); lanes 7 to 9, 3 CD3⁻ LDGL (cases no. 10 to 12 in Table 1).