Binding of ABL proteins to bacterially expressed CRKL. Lysates from 1 × 10⁷ 32Dp210 cells were analyzed for binding to GST, glutathione beads (beads), or the various GST-CRKL constructs as described in Materials and Methods. Bound proteins were separated by SDS-PAGE and transferred to PVDF membranes. ABL proteins were detected using the 24-21 ABL MoAb. Molecular weight markers are indicated on the left side of the panel.