Binding of BCR-ABL proteins to bacterially expressed CRKL. Lysates from 32D, 32Dp210, or 32Dp210ΔP1,P2 cells were analyzed for binding to (A) GST-SH3n or (B) GST-CRKL. Bound proteins were separated by SDS-PAGE and transferred to PVDF membranes. ABL proteins were detected using the 24-21 ABL MoAb. The 32Dp210 and 32Dp210ΔP1,P2 lysates were normalized for BCR-ABL expression before this analysis. Molecular weight markers are indicated on the left side of the panel.