Binding of cellular proteins to bacterially expressed CRKL. Lysates from 1 × 10⁷ 32Dp210 cells were bound to GST, glutathione beads (beads), or the various GST-CRKL constructs as described in Materials and Methods. Bound proteins were separated by SDS-PAGE and transferred to PVDF membranes. (A) Tyrosine-phosphorylated proteins were detected with 4G10, a monoclonal antiphosphotyrosine antibody and (B) paxillin was detected with an anti-paxillin MoAb. Molecular weight markers are indicated on the left side of each panel. The lane marked 32Dp210 in (A) is a whole-cell lysate lane.