Detection by RT-PCR of the c-fes mRNA in FDC-P1/MAC-11 cells treated with c-fes ODN. Total cellular RNA was extracted after 5 days of murine c-fes AS ODN treatment as described in Materials and Methods. The 414-bp amplified murine c-fes fragment was obtained using the oligonucleotide primers mFES-DP (5′-CAGAGCTGGAGCAGCGGCCCCGACA-3′ ) and mFES-RP (5′-GGTGCAGCTGTGCGGCCCTCACACC-3′ ). The 131-bp murine β2-microglobulin fragment, used as a quantitative control, was obtained using the oligonucleotide primers mβ2m-DP (5′-GGTGCTTGTCTCACTGACCGGCTT-3′ ) and mβ2m-RP (5′-GAGGCGGGTGGAACTGTGTTACGT -3′ ); lane 1, DNA mol wt marker VI (Boehringer); lane 2, FDC-P1/MAC-11 cells treated with the mixture of mFES-AS1, 2, and 3; lane 3, FDC-P1/MAC-11 cells treated with the mixture of mFES-IP1, 2 and 3; lane 4, ODN untreated FDC-P1/MAC-11 cells; lane 5, negative control performed with NIH-3T3 cells not expressing the c-fes gene; lane 6, proliferating FDC-P1/MAC-11 cells; lane 7, negative control performed without the cDNA template.