Fig. 3.
Detection by Western blot analysis of p92c-fes protein in HL60 cells treated with c-fes ODN. p92c-fes was monitored in HL60 cells after 3 days (A) and 5 days (B) of c-fes ODN treatment, whereas the level of expression of hck protein was evaluated only after 5 days of treatment (C). Lane 1, HL60 cells treated with the mixture of hFES-S-sj1 and 2; lane 2, HL60 cells treated with the mixture of hFES-AS-sj1 and 2; lane 3, HL60 cells treated with the mixture of the hFES-S1, 2, and 3; lane 4, HL60 cells treated with the mixture of hFES-AS1, 2, and 3; lane 5, HL60 cells treated with the mixture of hFES-IP-sj1 and 2; lane 6, HL60 cells treated with the mixture of the hFES-IP1, 2, and 3; lane 7, ODN untreated HL60 cells; lane 8, negative control performed with K562 cells not expressing the p92c-fes (A and B); (C) shows the hck expression in HL60 cells treated with all-trans retinoic acid (positive control) as already described.8