Flow cytometric cell cycle analysis of HL60 cells treated with c-fes ODN before and after induction for 48 hours with PMA. In each panel, the rectangles marked R2, R3, and R4 represent the cells in the Go/G1, S and G2/M phases of the cell cycle, respectively. (A) hFES-IP-sj-treated HL60 cells: the percentage of the cells in each phase of the cycle is 42% (Go/G1), 49% (S) and 9% (G2/M), respectively. Superimposable percentages were obtained in untreated, AS and control ODN treated cells, in several different experiments (data not shown). (B) hFES-IP-sj-treated HL60 cells induced with PMA; the percentage of the cells in the G1 phase of the cycle is 79%, in S phase 14% and in G2/M phases 7%. (C) Distribution of HL60 cells in the different phases of the cycle after 5 days of hFES-AS-sj1 and 2 ODN and PMA treatment. The percentage of the cells in the G1 phase is 50%, in S is 42% and in G2-M is 8%. (D) Superimposable results to that described in (C) were obtained when HL60 cells were treated with the mixture of hFES-AS1, 2, and 3 and induced to macrophage differentiation.