Flow cytometric cell cycle analysis of FDC-P1/MAC-11 cells treated with c-fes ODN before and after induction for 48 hours with PMA. In each panel, the rectangles marked R2, R3, and R4 represent the cells in the Go/G1, S and G2/M phases of the cell cycle, respectively. (A) ODN untreated FDC-P1/MAC-11 cells: the percentage of the cells in each phase of the cycle is 47% (Go/G1), 44% (S) and 9% (G2/M), respectively. Superimposable percentages were obtained in c-fes mIP1, 2, and 3 ODN treated cells, in several different experiments (data not shown). (B) ODN untreated FDC-P1/MAC-11 cells induced with PMA; the percentage of the cells in the G1 phase of the cycle is 79%, in S phase 10%, and in G2/M phases 11%. (C) Distribution in the cell cycle phases of mIP ODN treated FDC-P1/MAC-11 cells after PMA treatment. The percentage of the cells in the G1 phase is 79%, in S is 11%, and in G2-M is 10%. (D) Superimposable results to that described in (A) were obtained when FDC-P1/MAC-11 cells were treated with the mixture of mFES-AS1, 2, and 3 and induced to macrophage differentiation with TPA (49% in G1, 43% in S, and 8% in G2-M).