Electrophoretic mobility of glycosidase-treated factor V. (A) Purified factor V on 5% SDS-PAGE stained with Coomassie blue G-250. Native factor V (lane 1); neuraminidase-treated factor V (lane 2); neuraminidase, O-glycanase–treated factor V (lane 3); neuraminidase, N-glycanase–treated factor V (lane 4); and neuraminidase, O-glycanase, N-glycanase–treated factor V (lane 5). (B) Silver-stained 5% SDS-PAGE analysis of thrombin-activated factor V samples described in (A).