Transactivation of the wild-type (WT) and mutant (−61 T to G) factor VII promoters by HNF-4 in HeLa cells. Growth hormone reporter plasmids containing the wild-type or mutant promoter element were transfected with the expression vector pLEN4S that codes for HNF-4 or the empty pLEN basic vector. A β-galactosidase plasmid was also cotransfected to correct for variations in transfection efficiency. The results are expressed in ng/mL of secreted growth hormone and represent the mean ± 1 SD of three independent transfections. HNF-4 transactivated the wild-type factor VII promoter 5.4-fold, while no effect was seen with the mutant promoter.