Fig. 2.
![Fig. 2. Tyrosine phosphorylation of platelet polypeptides in the presence of two competitive inhibitors of αIIbβ3, the RGDS peptide and the monoclonal antibody 10E5. Platelets were incubated with 1 mmol/L RGDS peptide (A) or 20 μg/mL 10E5 monoclonal antibody (ANTIαIIbβ3, [B]) before stimulation with 0.05 U/mL thrombin for 0, 30, 60, 120, or 240 seconds. Solubilized proteins were then analyzed by Western blotting as in Fig 1. Aggregation rates are indicated. src, the 64 kD, the 77 to 80 kD, and 100 to 105 kD doublets, and the 130 kD band are indicated. These autoluminograms are representative of three separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/12/10.1182_blood.v89.12.4385/3/bl_0007f2.jpeg?Expires=1735571589&Signature=Np5UWxTqPx2-Uvny5t~RBFnkqBc7S1oYuSynvGP4RrD3NOrr~WmwFsYN5dD4t1Qn75gqArofgPUv~F1WawrTFeoNA5vcklYg-mJhK5bp1KoTkxosVmRr6v~mswWsCflmK44yA8hEppp06bqQaiNbwAodAcbaPZnS5ifvPJdiLyv9uDrI0IzgTLpkMShRdek3fynNSRAN1E0TG~QFIAcIqpulfxMH3QZHwKt9j9uN64k5NqKxfDFqymfKR0l1RuUSoXZko43GPGqP11zYRwmMz6G~RTHC8Fbvf1bOCK61U2Ms3UWBTLzoGNgHMAiE0W3mO4TXpAqpPwdIFS1UFdyYKg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Tyrosine phosphorylation of platelet polypeptides in the presence of two competitive inhibitors of αIIbβ3, the RGDS peptide and the monoclonal antibody 10E5. Platelets were incubated with 1 mmol/L RGDS peptide (A) or 20 μg/mL 10E5 monoclonal antibody (ANTIαIIbβ3, [B]) before stimulation with 0.05 U/mL thrombin for 0, 30, 60, 120, or 240 seconds. Solubilized proteins were then analyzed by Western blotting as in Fig 1. Aggregation rates are indicated. src, the 64 kD, the 77 to 80 kD, and 100 to 105 kD doublets, and the 130 kD band are indicated. These autoluminograms are representative of three separate experiments.
