Fig. 5.
Graphical representation of the in vivo binding affinities of blood cells and spleen tissue following IV administration of 125I-rmTPO. Mice received a single IV bolus injection of 125I-rmTPO or 125I-rmTPO + increasing concentrations of rmTPO and were killed 2 hours later. Citrated blood and spleens were procured and blood cell and spleen-associated radioactivity were determined. The binding affinities were determined by 4 parameter fits of % blood cell-associated (A) and spleen-associated cpm/gram (B) versus dose of rmTPO expressed in μg/kg.
