Hematologic effects in the femur 48 hours after the administration of a single intravenous dose of doxorubicin (doxo; 17.5 mg/kg). See legend to Fig 2 for key to other abbreviations. (A) Effects of doxorubicin alone. In the middle panel, note that doxorubicin depletes clonogenic progenitors as efficiently as nonclonogenic BM MNCs, leading to a profound reduction in total progenitor numbers as shown in the lower panel. (B) Preceding SCF administration does not significantly alter doxorubicin-mediated BM toxicity. The inability of SCF to significantly augment doxorubicin's effect on progenitors is shown in (C). Error bars in (A) and (B) middle and lower panels designate standard deviations.