![Fig. 1. SDS-PAGE analysis of 35S-labeled rPLG immunoprecipitated from the culture media of transfected COS-1 cells. (A) COS-1 cells were transiently transfected with pTH3/WT (WT) or pTH3/S572P (S572P) plasmids. Cells were metabolically labeled with L-[35S] methionine, and the culture media (1.5 mL) was immunoprecipitated with goat polyclonal anti-human PLG (IgG fraction). Immunoprecipitation products were analyzed by 10% SDS-PAGE and autoradiography under nonreducing conditions. The migration of protein standards is indicated. (B) Intracellular PLG purified from HepG2 cells (CE) and protein A-Sepharose beads containing adsorbed rPLG (CM) were treated with (+) or without (−) endo H as described in Materials and Methods. Each sample was boiled without a reducing agent, centrifuged, and the supernatant was subjected to 10% SDS-PAGE as described above.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/1/10.1182_blood.v89.1.183/2/bl_0016f1.jpeg?Expires=1736051818&Signature=IPwMrvIl8pmzf-ugeCltx3Cz1XWQFei3jwXshHm~Im1Sl8FfWBPRW8eVBTl~Jpsq1xYqbXX84wSkpTzq6ZB0tnBOBdy7TrdPs~fbn30IrZdc3cbP0HnJVSXqOzFvo4VubapoEcKHpwxmTtP4Gpeoc1Pca2CSJrGpKgAWIse9Y3LaDS6Bco60v6NT3G7Fr7aAQX1Z0JlNiGPdDaLY7t99trZGJmuzrNE4TlkqauHXXjGrHuB06Ts25HJqd24OLUWZqakkUOGIsv5BEzlp2~7u3Mq7yH0MDTy45aaHOo~1IC-5WNlitq8kNORFr9cHiQJyVB-09cP3m~mX1Ci6O-7xsQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
SDS-PAGE analysis of 35S-labeled rPLG immunoprecipitated from the culture media of transfected COS-1 cells. (A) COS-1 cells were transiently transfected with pTH3/WT (WT) or pTH3/S572P (S572P) plasmids. Cells were metabolically labeled with L-[35S] methionine, and the culture media (1.5 mL) was immunoprecipitated with goat polyclonal anti-human PLG (IgG fraction). Immunoprecipitation products were analyzed by 10% SDS-PAGE and autoradiography under nonreducing conditions. The migration of protein standards is indicated. (B) Intracellular PLG purified from HepG2 cells (CE) and protein A-Sepharose beads containing adsorbed rPLG (CM) were treated with (+) or without (−) endo H as described in Materials and Methods. Each sample was boiled without a reducing agent, centrifuged, and the supernatant was subjected to 10% SDS-PAGE as described above.
