Fig. 2.
SDS-PAGE analysis of stable transfectants secreting the activation-resistant rPLG. Stable transfectants secreting wild-type (R561S) and mutant (R561S + S572P) rPLGs were established in CHO-K1 cells and labeled with L-[35S] methionine at the time of 80% confluency. Immunoprecipitation products were electrophoresed under nonreducing conditions by 10% SDS-PAGE.