Fig. 3.
![Fig. 3. SDS-PAGE analysis of 35S-labeled rPLG immunoprecipitated from the culture media of transfected COS-1 cells. COS-1 cells were transiently contransfected with pTH3/WT (WT) or pTH3/R561S (R561S) and pSV–β-galactosidase plasmids. The cells were metabolically labeled with L-[35S]-methionine, and the culture media (1.5 mL) was immunoprecipitated with goat polyclonal anti-human PLG (IgG fraction) and rabbit polyclonal anti–β-galactosidase (IgG fraction). The immunoprecipitation products were analyzed by 10% SDS-PAGE and autoradiography under nonreducing conditions. The migration of protein standards and β-galactosidase is indicated.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/1/10.1182_blood.v89.1.183/2/bl_0016f3.jpeg?Expires=1736052419&Signature=0pNe3qCLSST4TTBa2n-Q1b7PWsrsKtIha~t2cIhiZb9OE1E~6Tl7gaVevRersrU9A1nCzHiuOovV7hlFcKg93TlkUG~IqpVZYSxoc7ppGm15JGTQPhk6BVEZLrfgkXhNJktZQ2LyCTfymGLWKpJCwKWTjK8YEthbJnxpSa36jv99peD~Iexsd3A2gl4RXuQtWwxwhMbzVCjtkm2qXyOKqqwAMSAYZD9ZlUeAH~GmJrRtIwaOEP1Zls13xZcT~NgotLvRrjVJE3SKX3z5ClCwQ1GR-tp5a8Vu6jFdRhqBBvNi0El98S-WcykwAnmmRFzfyAZtVIyXVc~hXGCVFeoAEw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
SDS-PAGE analysis of 35S-labeled rPLG immunoprecipitated from the culture media of transfected COS-1 cells. COS-1 cells were transiently contransfected with pTH3/WT (WT) or pTH3/R561S (R561S) and pSV–β-galactosidase plasmids. The cells were metabolically labeled with L-[35S]-methionine, and the culture media (1.5 mL) was immunoprecipitated with goat polyclonal anti-human PLG (IgG fraction) and rabbit polyclonal anti–β-galactosidase (IgG fraction). The immunoprecipitation products were analyzed by 10% SDS-PAGE and autoradiography under nonreducing conditions. The migration of protein standards and β-galactosidase is indicated.
