Fig. 5.
Pulse-chase analyses of 35S-labeled rPLG. Radiolabeled rWT (○) and rS572P (•) in culture medium (A) and cell extract (B) were quantitated by a laser densitometer. The quantity of β-gal immunoprecipitated at each time point was used to normalize both the transfection efficiency and the immunoprecipitation efficiency. Within each pulse-chase experiment, the number of counts is expressed in arbitrary units.
