American Society of Hematology

Fig. 3.

$Fig. 3. PCR before and after CD34 selection. Cells were analyzed for EGF-R expression before and after CD34 selection. Results from several patients are depicted. Lanes 1, 3, and 5, expression of EGF-R in LP. Lanes 2, 4, and 6, EGF-R expression in CD34+ fractions. Lanes 1 and 2, patient no. 6 first induction cycle. Lanes 3 and 4, patient no. 12 first analysis. Lanes 5 and 6, patient no. 12 second induction cycle. MW, molecular weight marker. Breast cancer cell line Cal 51 was used as positive control: β2-microglobulin served as a control.$

PCR before and after CD34 selection. Cells were analyzed for EGF-R expression before and after CD34 selection. Results from several patients are depicted. Lanes 1, 3, and 5, expression of EGF-R in LP. Lanes 2, 4, and 6, EGF-R expression in CD34⁺ fractions. Lanes 1 and 2, patient no. 6 first induction cycle. Lanes 3 and 4, patient no. 12 first analysis. Lanes 5 and 6, patient no. 12 second induction cycle. MW, molecular weight marker. Breast cancer cell line Cal 51 was used as positive control: β2-microglobulin served as a control.

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