Fig. 6.
![Fig. 6. Immunoprecipitation of cytokine-treated HUVEC by the β3 VNR antibody 10E2 and by the VNR complex antibody LM609. HUVEC were pretreated with IFN-γ and TNF-α and metabolically labeled with [35S] methionine and [35S]cysteine for the last 17 hours, as described. Immunoprecipitation of untreated HUVEC was performed on equivalent amounts of cell lysate with LM609 (lane 1) or the anti-β3 MoAb 10E2 (lane 2). TNF-α–treated (lane 3) and TNF-α +IFN-γ–treated (lane 4) HUVEC were immunoprecipitated with 10E2. The αv and β3 bands, which migrated at Mr = 140,000 and 110,000, respectively, are indicated by the arrows.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/11/10.1182_blood.v89.11.4071/4/bl_0033f6.jpeg?Expires=1735834216&Signature=sBd3Dpyz7g0hvPFab02kbnljW4ZlYCIxIJTFsFd7XVxBQD1-5OyStv39jOKZN2~X0zSyVmL-x4u2H~-Yj~2cCCJr4gK5oL07olyEIk20~6fhhydxAepTdpeKV0PwGzWgFzY0FQ52cAlIFbopmOdvsIVFlQV8aLx1Mri0QjBFAgkTP4sZmT9VQALXjL2oIb6fVBEksd-q22GJd7y1fO~KUGfLmB6Xc5s1z9FRgJfLWuNxM1-OKduId19fBxaYH~hrnlsQR0O-nC1H-EQTLV9Ke6CZHOGbYO6XvITmExsctMUblKe9vofcdzbV6hxlt~Vwqs2aSIllfC6iRN3yrNEKeQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunoprecipitation of cytokine-treated HUVEC by the β3 VNR antibody 10E2 and by the VNR complex antibody LM609. HUVEC were pretreated with IFN-γ and TNF-α and metabolically labeled with [35S] methionine and [35S]cysteine for the last 17 hours, as described. Immunoprecipitation of untreated HUVEC was performed on equivalent amounts of cell lysate with LM609 (lane 1) or the anti-β3 MoAb 10E2 (lane 2). TNF-α–treated (lane 3) and TNF-α +IFN-γ–treated (lane 4) HUVEC were immunoprecipitated with 10E2. The αv and β3 bands, which migrated at Mr = 140,000 and 110,000, respectively, are indicated by the arrows.
