Flow cytometry of surface-marker expression on macrophages generated from monocytes cultured with M-CSF or M-CSF + IL-10. Monocytes were incubated with M-CSF (50 ng/mL) + IL-10 (25 ng/mL) or M-CSF alone for 7 days. These cells were then stained for HLA-DR, CD14, CD16, CD32, CD64, CD11b Ag as described in Materials and Methods. Macrophages cultured in M-CSF alone are represented by a solid line, and those cultured in M-CSF + IL-10 are shown as dotted lines. Data are from representative experiment of three performed.