Fig. 1.
![Fig. 1. (A) 7C11 anti-Fas MoAb induces DNA fragmentation in RPMI 8226 MM cells. Cells were cultured in media alone and for the indicated times with 5 μg/mL of 7C11 anti-Fas MoAb. Genomic DNA was isolated, end-labeled with 0.5 mCi of [γ32-P]-dCTP, and analyzed by 1.8% agarose gel electrophoresis. MW, molecular weight markers (123-bp ladders and HindIII digest of l-DNA). (B) Quantification of anti-Fas-MoAb induced apoptosis in RPMI-8226 MM cells was performed by using propidium iodide staining technique. Cells were treated with anti-Fas MoAb for the indicated time intervals and stained with propidium iodide; percentage of apoptotic cells was analyzed by FACS. “C” represents control cells.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/1/10.1182_blood.v89.1.227/2/bl_0027f1.jpeg?Expires=1735538118&Signature=E0moUWJIDv9nsxDpIKxJ8s1sfK84joTNxEnICayuY39tmm8D7QDXAKOsjdqhFTBAgX524a4WnhhtZulYUd7NW39H0yJFagGfGpf1PNEyBNUQUEeGWhKdnU8NdpGADxRHvK0NHUbXgw0vRxz9O8WXw7TMXocq2JOFAVBh-Ytt8iYtqPJ7kI58yP2bYgIGJlpX1I0MgDKTRDSh1qTxvq1vkav7RpmdI2quy0dx7y3k8hbvfz3mczvCmhcnrp1Wi3aut0-7k9EOI17F~9WUAljRa9yP~dz1SPPAKwlcQR7NqKga2gH2n9SxO9tKc2rRfR1cL-tpedZd8E0koLCHdQiIfw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
(A) 7C11 anti-Fas MoAb induces DNA fragmentation in RPMI 8226 MM cells. Cells were cultured in media alone and for the indicated times with 5 μg/mL of 7C11 anti-Fas MoAb. Genomic DNA was isolated, end-labeled with 0.5 mCi of [γ32-P]-dCTP, and analyzed by 1.8% agarose gel electrophoresis. MW, molecular weight markers (123-bp ladders and HindIII digest of l-DNA). (B) Quantification of anti-Fas-MoAb induced apoptosis in RPMI-8226 MM cells was performed by using propidium iodide staining technique. Cells were treated with anti-Fas MoAb for the indicated time intervals and stained with propidium iodide; percentage of apoptotic cells was analyzed by FACS. “C” represents control cells.
