Multilineage engraftment in the BM of a mouse transplanted with CB cells at limiting dilution. BM from a mouse transplanted with 1 × 106 CB cells and killed after 6 weeks was stained with human-specific monoclonal antibodies and analyzed by flow cytometry. (A) Histogram of pan-leukocyte marker CD45 expression demonstrating 3% human cell engraftment in this mouse. (B) Cells for analysis were acquired in a live gate (R1) based on CD45 positivity and medium to high forward scatter. (C) Cells were further gated based on forward and side scatter properties into lymphoid/blast (R2) and myeloid (R3) windows. (D) Isotype control for nonspecific IgG staining of cells in R2. (E) Expression of CD38 and CD19, a pan-B-cell marker, on cells in R2. (F ) Isotype control for cells in R3. (G) Expression of myeloid marker CD33 and monocytic marker CD14 on cells in R3. (H) Expression of myeloid marker CD13 and mature granulocyte marker CD15 on cells in R3.
