Mixed or blast-type hematopoietic colonies derived from wild-type and GATA-2^−/− ES cells in the 2-step in vitro differentiation assay. Day 9 EBs were disaggregated and plated into secondary plates with medium containing KL, IL-3, IL-11, and Epo. Mixed and blast-type hematopoietic colonies of wild-type origin (A and C) or GATA-2^−/− (B and D) origin were examined on day 9 (A and B) and day 13 (C and D). This finding was reproduced in 2 experiments using 2 independent GATA-2^−/− clones.