Primitive erythroid, definitive erythroid, and macrophage colonies derived from wild-type and GATA-2^−/− ES cells in the 2-step in vitro differentiation. Day 9 EBS were disaggregated and plated into secondary plates with medium containing KL, IL-3, IL-11, and Epo. Colonies with cells of single lineages of wild-type origin (A, C, and E) and GATA-2^−/− origin (B, D, and F ) were compared. Primitive erythroid colonies (A and B), definitive erythroid colonies (C and D), and macrophage colonies (E and F ) were examined on days 5, 6, and 13, respectively.