![Fig. 5. Effect of Tpo on megakaryocytic differentiation. (A) UT-7 cells were cultured with either Tpo alone or the combination of nocodazole and Tpo as follows. [1] The cells were cultured for 2 days in the presence of 0.1% DMSO, which was used as a solvent for nocodazole. The medium was then replaced with those containing Tpo at the concentration of 10 ng/mL, and the culture was continued for an additional 5 days. [2] The cells were treated with Tpo for 2 days, and the medium was replaced with those containing 0.1% DMSO. The culture was continued for additional 5 days. [3] The cells were treated with 50 ng/mL of nocodazole for 2 days, and the medium was replaced with those containing Tpo. The culture was continued for an additional 5 days. (B) Wright-Giemsa staining of UT-7 cells was performed after the culture with three different conditions as shown above. (Original magnification × 400). (C) The intracellular concentration of β-TG was determined by ELISA after the culture in each condition. The mean ± SD (bar) of three independent experiments is shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/11/10.1182_blood.v89.11.3980/4/bl_0005f5.jpeg?Expires=1735536595&Signature=vAKEl4-VPix1gBofrtsFuET6NCSzgfl7BH6pA8eD9t1OAj2J3V4WIXF1jEU~vkz72NGqP0uVqpqGuwuUkg9Ckhj9EytW~X3JYXTSOXusFke5ClKzaCrXiMxImaToDnavsPb51bYgCmMGf-SdrDAHGSuVKnv3PDFEYC9-HvckHIuBwJi5ppzsiL2UoO6rvZNwh1sk~SFvhBTjc0vV-kJZX37D~ud3W9E7nVbtKcqi63WsBjfgNMTbVxAlgS5ioXw5fZo4I4uR3EhDxnCoWhfUvPevTKStTfaHEgC7vgy5ccNdsbnFqa1X2EPx0TJNP~IiIGgLh-3JIGwJAJ1IXX25jQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of Tpo on megakaryocytic differentiation. (A) UT-7 cells were cultured with either Tpo alone or the combination of nocodazole and Tpo as follows. [1] The cells were cultured for 2 days in the presence of 0.1% DMSO, which was used as a solvent for nocodazole. The medium was then replaced with those containing Tpo at the concentration of 10 ng/mL, and the culture was continued for an additional 5 days. [2] The cells were treated with Tpo for 2 days, and the medium was replaced with those containing 0.1% DMSO. The culture was continued for additional 5 days. [3] The cells were treated with 50 ng/mL of nocodazole for 2 days, and the medium was replaced with those containing Tpo. The culture was continued for an additional 5 days. (B) Wright-Giemsa staining of UT-7 cells was performed after the culture with three different conditions as shown above. (Original magnification × 400). (C) The intracellular concentration of β-TG was determined by ELISA after the culture in each condition. The mean ± SD (bar) of three independent experiments is shown.
