TFPI inhibits PMN adhesion in response to endotoxin. Fluorescently labeled PMN were incubated for 10 minutes 37°C in the presence of stimuli and the indicated concentrations of rTFPI. After washing, adhesion to fibrinogen-coated surface was measured. Stimuli were LPS (10 ng/mL, Rb from E coli K12) with NHP (2%; •), LPS with rLBP (1 μg/mL; ▴), rTNF-α (0.5 ng/mL; ▾), and buffer alone (▪) in the presence of increasing doses of rTFPI. The average of triplicates and standard deviation are shown as a symbol and error bars. (♦) A PMN sample incubated with LPS only. Data are plotted on logarithmic X scale.