Fig. 2.
![Fig. 2. Inability of GM-CSF/IFN-γ–treated (HLA-DR+) PMN to stimulate TT-specific T cells. Purified monocytes (Mφ; 5 × 104/well) were used as control APC. PMN were first incubated for 24 hours in the presence of GM-CSF/IFN-γ (50 U/mL), and then added to wells of a 96-well plate at concentrations of 0, 1.25, or 2.5 × 105 cells/well. TT-specific T cells were than added, and the Ag presentation assay performed as described in Materials and Methods. [3H]TdR uptake was measured at 72 to 96 hours. Data are expressed as cpm ± SD of duplicate samples.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/11/10.1182_blood.v89.11.4128/4/bl_0007f2.jpeg?Expires=1735848076&Signature=tFnr1SM58vGvJ2P5EFBAoOuvXL~xx2rJr~zdJThekt3YW~XVJG2azmSoGdNDyeQNxTf9A5TsFt4LNUVnabVhWdLonjNxA1L8IuMEwCOQbEezCR7ILQpUjdaME8~YuTY74YwBJvpIf508s8l3KrvFHUuuMtE~K8yWVD5zZNzw4vsOIkzpM8DUeFgOlYxGWSrxs3hlh9N2rJXGV~h0SOqAklIm6rrqNseziPCiLjbsLCT1MZCmeOuYoncjssN6BMI29Ye0XGdX7ySnUxw90mih1gZqKCt4oB-YjH0FmXqHIKAuC07oGwx~6ze1xjYKrpjcghE1aN0~duyjySdH1hSuxw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inability of GM-CSF/IFN-γ–treated (HLA-DR+) PMN to stimulate TT-specific T cells. Purified monocytes (Mφ; 5 × 104/well) were used as control APC. PMN were first incubated for 24 hours in the presence of GM-CSF/IFN-γ (50 U/mL), and then added to wells of a 96-well plate at concentrations of 0, 1.25, or 2.5 × 105 cells/well. TT-specific T cells were than added, and the Ag presentation assay performed as described in Materials and Methods. [3H]TdR uptake was measured at 72 to 96 hours. Data are expressed as cpm ± SD of duplicate samples.
