Fig. 3.
![Fig. 3. Inability of GM-CSF/IFN-γ–treated (HLA-DR+) PMN to stimulate TT peptide-specific T cells. Flow cytometric analysis of PMN treated with GM-CSF/IFN-γ (50 U/mL) for the presence of HLA-DR (inset) was conducted as described in Materials and Methods. The open peak represents staining with the isotype control. The closed peak represents staining with HLA-DR–specific antibody. T-cell stimulation was conducted as described in Fig 2, but using 5 μg/mL TT peptide instead of whole TT. [3H]TdR uptake was measured at 72 to 96 hours. Data are expressed as cpm ± SD of triplicate samples.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/11/10.1182_blood.v89.11.4128/4/bl_0007f3.jpeg?Expires=1735847586&Signature=AQt6eFB-0vtkKmOO6e2F5BOYF12VacO3kLnaOJSA7D4XFMrNJcxqOQ7Q2OQBKJl3f4VIG2psSjdiQHAmqFuVAV8hSVl7-gx-dh-GazWgsYUk5InRHQWdsXddaNF9auvBFy7Mtu-UiyS085nJQSskucjVCEJeJEojjTHRP2rIP7e3CE-2nMDRrW71T04wBzF6vAPioyY8fiD~JaDuevAeIDrfxP5p5zCSIu8zuzvBZ2hA4Z8wJ16KMWbXw24mR0zCPhbZvptD5UMgyScyitEQ4LkKNGXJVL4V4iPCBZzRaF59x~R-QvKGXCQuWD9GCUPzVHlqeKF5XzKD813adbsfQA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inability of GM-CSF/IFN-γ–treated (HLA-DR+) PMN to stimulate TT peptide-specific T cells. Flow cytometric analysis of PMN treated with GM-CSF/IFN-γ (50 U/mL) for the presence of HLA-DR (inset) was conducted as described in Materials and Methods. The open peak represents staining with the isotype control. The closed peak represents staining with HLA-DR–specific antibody. T-cell stimulation was conducted as described in Fig 2, but using 5 μg/mL TT peptide instead of whole TT. [3H]TdR uptake was measured at 72 to 96 hours. Data are expressed as cpm ± SD of triplicate samples.
