Fig. 7.
![Fig. 7. PMN SAg-induced T-cell proliferation is HLA-DR–restricted. PMN from both an HLA-DRlow responder (donor 7; , unstimulated; □, GM-CSF/IFN-γ) and HLA-DRhigh responder (donor 2; ▨, unstimulated; ▪, GM-CSF/IFN-γ) were cultured for 44 hours in the absence or presence of 100 U/mL GM-CSF/IFN-γ, and 1 × 105 PMN from each condition were incubated with 5 × 104 CD4+ T lymphocytes in the presence of 1 nmol/L SEA. The MoAbs L243 (anti–HLA-DR), 197 (anti-CD64), and AML223 (anti-CD14) were added to the appropriate wells 30 minutes before the addition of SAg. T-cell proliferation was measured at 66 hours with an 18- to 20-hour [3H]TdR pulse and is presented as counts per minute (cpm). Values represent the mean of triplicate samples ± SD.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/11/10.1182_blood.v89.11.4128/4/bl_0007f7.jpeg?Expires=1735846764&Signature=AUKJftiUibdrMNyAjYUsZ0gS2v6DEgMFtvCcDnFxacVsIzQD4SFtUusroO2fNEnvCUUiSBRNNvMtvy8hB3YDsep4Vz5g5QFCMWVrYDb~qNP2c0vQsYNPsPZV2s6eNPCsL-gVKeLrV9Ntzd5uObFeMlRPEZ2SZWfeKkKlOLuE0pDYZ4fzSsLqfURVaL63Ij1d1HjLdqkjgHd3ZQkmf6lczpvmS5WB~uW6ZXWNFZ~OlfrjGDAakRbhAd3gLdaBalLvqVWmEZIjppAZf-QFV3SDCc9-BjdFLZIA7o74b3PFMlhbpyTxGYr6beoUeaGEoZk2WzT8g0hUYfkDOGH1fkpNSw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PMN SAg-induced T-cell proliferation is HLA-DR–restricted. PMN from both an HLA-DRlow responder (donor 7; , unstimulated; □, GM-CSF/IFN-γ) and HLA-DRhigh responder (donor 2; ▨, unstimulated; ▪, GM-CSF/IFN-γ) were cultured for 44 hours in the absence or presence of 100 U/mL GM-CSF/IFN-γ, and 1 × 105 PMN from each condition were incubated with 5 × 104 CD4+ T lymphocytes in the presence of 1 nmol/L SEA. The MoAbs L243 (anti–HLA-DR), 197 (anti-CD64), and AML223 (anti-CD14) were added to the appropriate wells 30 minutes before the addition of SAg. T-cell proliferation was measured at 66 hours with an 18- to 20-hour [3H]TdR pulse and is presented as counts per minute (cpm). Values represent the mean of triplicate samples ± SD.
