Fig. 1.
![Fig. 1. Effect of IFN-γ on sphingomyelin (A) and ceramide (B) levels in HL-60 cells. [3H]Choline-labeled or unlabeled cells were treated at time 0 with 2,000 U/mL of IFN-γ. At the indicated times, cells were harvested, lipids were extracted, and SM levels were determined by TLC, while ceramide levels were determined using a DAG kinase assay as described in Materials and Methods. The data (means ± standard deviation [SD]) are from three independent experiments assayed in duplicate. * Significantly different (P < .05, Student's t-test) from controls.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/1/10.1182_blood.v89.1.81/2/bl_0028f1b.jpeg?Expires=1735533070&Signature=oTydN1LciXmT5oFiVq4AL-yq9OBv4sZvhgoFZ0in0MZB2onuSoiCJX8ldSiGNt2bOKeZ7-KphDehoK7e~YbVVxSPMd8ED8hPBZPOpK4RxBqBxGtr~xEDMhUth0vlK8WRMgslCP6UXIPsYt5flLhLbz15iWNSZx03dtmVhG54W6VppQhAgS0NDuLecWNX70bK8MtMrJHwn5JoaeIBCLxLmFYMxnk6y7lfj16Rh8ypkCi~grsDjdUjI472aiCn2gekNY-uDpRXzKzkNtfx1wJPEOMRxl-vlyzDeYocRl9KhpAhlDdteyxOa~47y3RAYM8rBV~XXRYkZua5dT1yUjUIaA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of IFN-γ on sphingomyelin (A) and ceramide (B) levels in HL-60 cells. [3H]Choline-labeled or unlabeled cells were treated at time 0 with 2,000 U/mL of IFN-γ. At the indicated times, cells were harvested, lipids were extracted, and SM levels were determined by TLC, while ceramide levels were determined using a DAG kinase assay as described in Materials and Methods. The data (means ± standard deviation [SD]) are from three independent experiments assayed in duplicate. * Significantly different (P < .05, Student's t-test) from controls.
