Fig. 2.
![Fig. 2. Time course and dose response curve of IFN-γ–stimulated AA release. HL-60 cells were prelabeled for 60 minutes with 5 μCi/mL [3H]AA, washed twice, and resuspended in the medium. After incubation with IFN-γ, cells were pelleted and the resultant supernatant was counted for 3H radioactivity. (A) Time course of AA release following treatment with 2,000 U/mL of IFN-γ. (B) Dose response of AA release at 2 minutes following IFN-γ–treatment. The data (means ± SD) are expressed as percentage of the control level obtained from three independent experiments assayed in duplicate. * Significantly different (P < .05, Student's t-test) from controls.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/1/10.1182_blood.v89.1.81/2/bl_0028f2b.jpeg?Expires=1735534043&Signature=FJMukZf~TUgBtTptqPskO59gJOhtsOSGH5nSjerbEMS49upClo37Ubvm5MNj7MToPvXuW-dtK1PutElW81sdCssOu7c48tE4iXCBvNXtA2wZJCU6Jp-LZVIdWMM8s8ikHlZOVxJ-VG-YYh7eDAQ7ua1zmt5RoXtKsKiedfljmL5bW193y38Fhp8fGf6lC-lGSZ2hP5QhkPvE8v9f7t1fxqQk3qhqo6fi7mI-5WHdF~NIElxg5X9mlP2u9lAQ1JxSyRw0XfWwVIJSRanc1Jq2VCLVrcyb-EnFWzM7sPe2xTIaXzt3fGjz9BS-yW22451bWFeWJ7Zh36Ny5ZiIwJEguA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Time course and dose response curve of IFN-γ–stimulated AA release. HL-60 cells were prelabeled for 60 minutes with 5 μCi/mL [3H]AA, washed twice, and resuspended in the medium. After incubation with IFN-γ, cells were pelleted and the resultant supernatant was counted for 3H radioactivity. (A) Time course of AA release following treatment with 2,000 U/mL of IFN-γ. (B) Dose response of AA release at 2 minutes following IFN-γ–treatment. The data (means ± SD) are expressed as percentage of the control level obtained from three independent experiments assayed in duplicate. * Significantly different (P < .05, Student's t-test) from controls.
