Fig. 3.
Fig. 3. PLA2 activity in membrane and cytosol fractions of IFN-γ–stimulated HL-60 cells. HL-60 cells were incubated with 2,000 U/mL of IFN-γ for indicated times and homogenized as described in Materials and Methods. PLA2 activity in particulate and soluble fractions was determined by hydrolysis of [arachidonyl-14C]PtdCho in the presence of 5 mmol/L DTT and 150 nmol/L Ca2+. (A) PLA2 activity in membrane fraction of HL-60 cells treated with 2,000 U/mL of IFN-γ for indicated times. (B) PLA2 activity in cytosol fraction of HL-60 cells treated with 2,000 U/mL of IFN-γ for indicated times. The data (means ± SD) are expressed as percentage of the control level obtained from three independent experiments assayed in duplicate. * Significantly different (P < .05, Student's t-test) from controls.

PLA2 activity in membrane and cytosol fractions of IFN-γ–stimulated HL-60 cells. HL-60 cells were incubated with 2,000 U/mL of IFN-γ for indicated times and homogenized as described in Materials and Methods. PLA2 activity in particulate and soluble fractions was determined by hydrolysis of [arachidonyl-14C]PtdCho in the presence of 5 mmol/L DTT and 150 nmol/L Ca2+. (A) PLA2 activity in membrane fraction of HL-60 cells treated with 2,000 U/mL of IFN-γ for indicated times. (B) PLA2 activity in cytosol fraction of HL-60 cells treated with 2,000 U/mL of IFN-γ for indicated times. The data (means ± SD) are expressed as percentage of the control level obtained from three independent experiments assayed in duplicate. * Significantly different (P < .05, Student's t-test) from controls.

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