LDF and MAP in rats systemically treated with L/D-NAME and infused with human RBCs. Washed human SS-RBCs or AA-RBCs (3 mL) were infused (time −18 to 0 minutes) into rats treated with either L-NAME or D-NAME (time −48 minutes) as described in Fig 3: L-NAME + AA-RBCs (LN-AA, n = 6), D-NAME + SS-RBCs (DN-SS, n = 5), and D-NAME + AA-RBCs (DN-AA, n = 3). The group of rats that received L-NAME and SS-RBCs was divided into two groups: (1) rats that survived the experiment (LN-SS alive, n = 4) and (2) rats that developed stroke and died (LN-SS stroke, n = 5). To allow for comparison with the other groups and statistical analysis, the time of stroke (LDF <10) for rats in the LN-SS stroke group was normalized to 20 minutes (median time of stroke); data collected for the 15 minutes immediately before the stroke was also normalized (normalized times of 5, 10, 15, 17, 19, and 20 minutes). The graphs represent the mean ± SE of the (A) LDF (percent change from baseline) and (B) MAP (mm Hg). †P < .01 for all L-NAME–treated groups compared with all D-NAME–treated groups. *P < .05 for LN-SS stroke group compared with LN-SS alive group. **P < .05 for LN-SS stroke group compared with all other groups. ¶P < .05 compared with time 0 within the LN-SS stroke group.