Nuclear association of ACHEmRNA in DAMI cells. High resolution in situ hybridization was performed on cells in suspension using a DIG-labeled cRNA probe. Note that the yellow fluorescent signal (marked by arrows in the top panel) appeared in the presence of probe around multiple lobes in both small (left-hand side) and large MK (right-hand side) and was less pronounced in the cytoplasm, particularly in large cells. The absence of signal in cells treated similarly, but with no probe in the hybridization solution (bottom panel), demonstrated that these fluorogenic signals reflect cellular mRNA.