Fig. 6.
Effect of A23187, serum depletion, and γ-irradiation on cell viability or induction of DNA fragmentation in EW-36 and JLP 119. Cells (5 × 105/mL) were treated with A23187 (1 or 2 μmol/L), serum depletion (reduced from 10% to either 1% or 0.2%), or γ-irradiation (600 or 1,200 rads). After 48 hours of incubation, cell viability was determined by trypan blue exclusion (A). Fragmentation of total cellular DNA (1 × 106 cells) was assessed by agarose gel electrophoresis (B). The viability results (▪, EW-36; , JLP 119) represent the mean ± SEM of quadruplicate (A23187) or duplicate (serum depletion and γ-irradiation) experiments. The DNA fragmentation data shown are from a representative study that was repeated two to four times.
