Quantitation of extent and type of H₂O₂ - and A23187-induced cell death induced in BL cell lines by Hoechst/propidium iodide nuclear staining. EW-36 and JLP 119 cells were treated for 24 hours with 100 μmol/L H₂O₂ or for 48 hours with 2 μmol/L A23187. Cell death (the percentage of propidium iodide-stained cells) and morphology were quantified as described in the Materials and Methods. The data represent the results from four separate experiments. (▪) Necrotic death; (▨) apoptotic death.