Flow cytometric TUNEL assay of JLP 119–bcl-2 transfectants treated with H₂O₂ or A23187. JLP 119 cells with a stable transfection of either control plasmid (G4 and G21) or plasmid expressing the human bcl-2 gene (H6 and H10) were tested for induction of TUNEL activity as described in the legends to Figs 7 and 10. (▨) H₂O₂ was added at 50 μmol/L and cells were assayed 24 hours later. (▨) A23187 was added 1 μmol/L and the cells were assayed 48 hours later. The data are taken from one representative experiment. (▪) No treatment.