Fig. 6.
![Fig. 6. Gelation concentration (Csat ) of HbS and E6V(β)/D85K(α). Oxy hemoglobin samples in 50 mmol/L potassium phosphate, pH 7.5, were mixed anaerobically with dextran and sodium dithionite, incubated at 37°C, and centrifuged. The hemoglobin concentrations in the supernatant before (initial [Hb]) and after (equilibrium [Hb]) the incubation were determined by amino acid analysis. If the equilibrium [Hb] is lower than the initial [Hb], it represents the gelation concentration of Csat of the Hb.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/11/10.1182_blood.v89.11.4196/4/bl_0023f6.jpeg?Expires=1735832486&Signature=JiUacl3UyPf6OEKWSXOSIvwFoReNHv2KGGmzz5XxfR3c5jtZCD9AnFimO7RdDMFLMG3tYYvDNpkQp3jH7nucVdJyBg90kQPMfG4Uh~vGr5NecMKs9NY9FzjrMs0ilUF3Zp0hbPjQfarq96pS-b97-YxhmVWsY0eRofodfYorlGbAbPDZhBtKL8C7AFXJmAsXiRM4sh7aWU27merkUFvtvARz5ko0Vy9uuE8hliPVz0FOTCAHiaEp3WHGq5-4BhBjv8CHjNp9Ho7UCncKHvW0o0uXDqNi02Etz-a-55RL8vgvLfHiFBA3QPyIGHzet6TUq1jmYpm5dNbOwf~Raa~TiA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Gelation concentration (Csat ) of HbS and E6V(β)/D85K(α). Oxy hemoglobin samples in 50 mmol/L potassium phosphate, pH 7.5, were mixed anaerobically with dextran and sodium dithionite, incubated at 37°C, and centrifuged. The hemoglobin concentrations in the supernatant before (initial [Hb]) and after (equilibrium [Hb]) the incubation were determined by amino acid analysis. If the equilibrium [Hb] is lower than the initial [Hb], it represents the gelation concentration of Csat of the Hb.
