Fig. 2.
Fig. 2. CPP32 immunostaining of normal and reactive lymph nodes and in B-cell malignancies. Some representative examples of the CPP32 immunostaining results are presented. (A) Normal node showing small secondary follicles with strong CPP32 immunostaining in germinal centers (GC) surrounded by a cuff of mantle zone (MZ) cells lacking CPP32 immunoreactivity and scattered cells in the interfollicular regions (IF ) with CPP32 immunopositivity (original magnification ×40). Inset shows same sample in which the CPP32 antiserum was first preadsorbed with CPP32 protein. (B and C) RFH (original magnification ×40 and ×200, respectively), showing enlarged follicles with strongly CPP32 immunopositive GCs, surrounding cuff of CPP32-negative MZ lymphocytes, and scattered cells in IF region with strong CPP32 immunoreactivity. In (C), the GC is located towards the right and the IF zone to the left. (D and E) Higher-power (original magnification ×1,000) views of CPP32-immunostaining in GC and IF zone, respectively, from a node with RFH. CPP32 immunostaining is found in cytosol or both cytosol and nucleus of some lymphocytes, particularly large noncleaved, transformed (“activated”) lymphocytes in the IF zone as well as in the plasma cells. Small round lymphocytes in the IF region are typically CPP32 negative or only weakly immunostained. In (F ), a multinucleated giant cell with moderate intensity cytosolic CPP32 immunostaining is shown, surrounded by mostly CPP32-negative small lymphocytes, in a case of RFH (original magnification ×1,000). (G through I) A case of FSC lymphoma at original magnification ×40, ×200, and ×400, respectively, showing CPP32-negative small cleaved cells filling centers of follicles, with residual strongly CPP32-positive normal transformed (activated) lymphocytes and plasma cells surrounding the follicles. In (I), the center of the follicle is oriented towards the right/bottom corner and a band of CPP32-positive normal cells located at the periphery of the follicle is shown in upper-left corner. (J through L) A case of FLCL immunostained for CPP32 (J and K) (original magnification ×40 and ×400) or Bcl-2 (L) (original magnification ×400) showing mixtures of large cells with strong cytosolic CPP32 immunostaining and small cleaved cells with little or no CPP32 immunoreactivity. The small cleaved cells, however, stain strongly for Bcl-2 in their cytosol (L). (M) DLCL showing large neoplastic cells with strong, mostly cytosolic CPP32 immunoreactivity (top) at the border with residual normal small, round lymphocytes (bottom) still present in the node (original magnification ×400). (N and O) MCL showing remnant of normal GC (located at center in N and in upper-right corner in O) with moderately CPP32-positive histiocytes and occasional adjacent large noncleaved cells with strong CPP32 immunoreactivity, surrounded by MCL cells that mostly contain no or only weak CPP32 immunoreactivity (original magnification ×200 and ×400). (P) Plasmacytoma, showing plasmacytoid cells with moderate to strong intensity CPP32 immunoreactivity, mostly in the cytosol (original magnification ×1,000). Red cells are immunonegative.

CPP32 immunostaining of normal and reactive lymph nodes and in B-cell malignancies. Some representative examples of the CPP32 immunostaining results are presented. (A) Normal node showing small secondary follicles with strong CPP32 immunostaining in germinal centers (GC) surrounded by a cuff of mantle zone (MZ) cells lacking CPP32 immunoreactivity and scattered cells in the interfollicular regions (IF ) with CPP32 immunopositivity (original magnification ×40). Inset shows same sample in which the CPP32 antiserum was first preadsorbed with CPP32 protein. (B and C) RFH (original magnification ×40 and ×200, respectively), showing enlarged follicles with strongly CPP32 immunopositive GCs, surrounding cuff of CPP32-negative MZ lymphocytes, and scattered cells in IF region with strong CPP32 immunoreactivity. In (C), the GC is located towards the right and the IF zone to the left. (D and E) Higher-power (original magnification ×1,000) views of CPP32-immunostaining in GC and IF zone, respectively, from a node with RFH. CPP32 immunostaining is found in cytosol or both cytosol and nucleus of some lymphocytes, particularly large noncleaved, transformed (“activated”) lymphocytes in the IF zone as well as in the plasma cells. Small round lymphocytes in the IF region are typically CPP32 negative or only weakly immunostained. In (F ), a multinucleated giant cell with moderate intensity cytosolic CPP32 immunostaining is shown, surrounded by mostly CPP32-negative small lymphocytes, in a case of RFH (original magnification ×1,000). (G through I) A case of FSC lymphoma at original magnification ×40, ×200, and ×400, respectively, showing CPP32-negative small cleaved cells filling centers of follicles, with residual strongly CPP32-positive normal transformed (activated) lymphocytes and plasma cells surrounding the follicles. In (I), the center of the follicle is oriented towards the right/bottom corner and a band of CPP32-positive normal cells located at the periphery of the follicle is shown in upper-left corner. (J through L) A case of FLCL immunostained for CPP32 (J and K) (original magnification ×40 and ×400) or Bcl-2 (L) (original magnification ×400) showing mixtures of large cells with strong cytosolic CPP32 immunostaining and small cleaved cells with little or no CPP32 immunoreactivity. The small cleaved cells, however, stain strongly for Bcl-2 in their cytosol (L). (M) DLCL showing large neoplastic cells with strong, mostly cytosolic CPP32 immunoreactivity (top) at the border with residual normal small, round lymphocytes (bottom) still present in the node (original magnification ×400). (N and O) MCL showing remnant of normal GC (located at center in N and in upper-right corner in O) with moderately CPP32-positive histiocytes and occasional adjacent large noncleaved cells with strong CPP32 immunoreactivity, surrounded by MCL cells that mostly contain no or only weak CPP32 immunoreactivity (original magnification ×200 and ×400). (P) Plasmacytoma, showing plasmacytoid cells with moderate to strong intensity CPP32 immunoreactivity, mostly in the cytosol (original magnification ×1,000). Red cells are immunonegative.

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