Flow cytometric characterization of day 5 MLR-generated CTLs (H-2^b anti–H-2^d) derived from granzyme B^+/+, granzyme B^−/−, and granzyme B^−/− × gld/gld spleens. CTLs were analyzed for CD3, CD4, and CD8 expression by flow cytometry. The relative number of live-gated cells (Y-axis) and logarithmic fluorescence intensity (X-axis) are plotted. All three types of CTLs have a similar composition of T-cell subsets, showing the normal ability of granzyme B−/− × gld/gld effectors to proliferate and activate in response to allogeneic stimuli in vitro.