Fig. 2.
Dose-response data of doxorubicin-treated Jurkat cultures at 24 hours. Jurkat cultures were treated with 0, 60, 80, 100, 200, 300, and 400 ng/mL of doxorubicin for 24 hours. The percentage of apoptotic cells in each culture is plotted against the CH2/CH3 signal intensity ratio determined by the 1H NMR spectra obtained at 400 MHz (64 excitations). The solid line and dashed lines are the best fit lines determined by linear regression analyses corresponding to the percentage of apoptotic cells as measured by morphologic analysis (r2 = .965) and FITC-annexin V flow cytometry (r2 = .985), respectively.
