American Society of Hematology

Fig. 3.

$Fig. 3. Dose-response data of doxorubicin-treated Jurkat cultures at 48 hours. Jurkat cultures were treated with 0, 25, 50, 100, 150, 200, and 400 ng/mL of doxorubicin for 48 hours. The percentage of apoptotic cells in each culture is plotted against the CH2/CH3 signal intensity ratio determined by the 1H NMR spectra obtained at 400 MHz (64 excitations). The solid line represents the best fit line determined by linear regression analysis corresponding to the percentage of apoptotic cells as measured by morphologic analysis (r2 = .964). The dashed line is the best fit line determined by linear regression analysis corresponding to the fraction of apoptotic cells determined by FITC-annexin V flow cytometry assay up to a dose of 150 ng/mL (r2 = .978).$

Dose-response data of doxorubicin-treated Jurkat cultures at 48 hours. Jurkat cultures were treated with 0, 25, 50, 100, 150, 200, and 400 ng/mL of doxorubicin for 48 hours. The percentage of apoptotic cells in each culture is plotted against the CH₂/CH₃ signal intensity ratio determined by the ¹H NMR spectra obtained at 400 MHz (64 excitations). The solid line represents the best fit line determined by linear regression analysis corresponding to the percentage of apoptotic cells as measured by morphologic analysis (r² = .964). The dashed line is the best fit line determined by linear regression analysis corresponding to the fraction of apoptotic cells determined by FITC-annexin V flow cytometry assay up to a dose of 150 ng/mL (r² = .978).

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