Thin-layer chromatograms of lipid extracts of apoptotic Jurkat T-cell cultures with different therapies. TLC was performed using the lipid extracts of untreated (control) and treated (apoptotic) Jurkat T-cell cultures. Three separate TLC are shown (ie, TLC #1, #2, and #3) each performed on different dates. Lipid standards (outside lanes) and lipid extracts from cell cultures (inner 3 lanes) were spotted and developed as described. TLC #1 (lanes 1A through 1E): 1A, standard; 1B, doxorubicin-treated cells; 1C untreated (control) cells; 1D, doxorubicin-treated cells; 1E, standard. TLC #2 (lanes 2A through 2E): 2A, standard; 2B, untreated cells; 2C, serum-starved cells; 2D, CH11 IgM anti-Fas antibody-treated cells; 2E, standard. TLC #3 (lanes 3A through 3E): 3A, standard; 3B, untreated cells; 3C, serum-starved cells; 3D, CH11 IgM anti-Fas antibody-treated cells; 3E, standard. Rf scale is the fractional height of spots relative to the total length of TLC plates from origin. Rf = 0.0 origin; Rf = 1.0 solvent front. Doxorubicin treatment (200 ng/mL for 24 hours); serum-starved cells (for 72 hours); CH11 IgM anti-Fas antibody treatment (200 ng/mL for 48 hours). Standards: Lyso LPC = lyso (mono) [12:0] PC; DMPS = di [14:0] PS; DOPC = di [18:1] PC; DLPE = di [12:0] PE; DPPC = di [16:0] PC; DSPC = di [18:0] PC. NB, [n₁ :n₂], where n₁ is the chain length of fatty acyl group and n₂ is the number of unsaturations of fatty acyl group. di, mono = number of acyl chains. PC, PS, and PE refer to type of head phospholipid group.