Cytotoxicity of T-cell clones against auto LCL cells and PHA-stimulated peripheral blood mononuclear cells (PHA-blasts). Auto LCL cells and peripheral blood mononuclear cells that were cultured with 1% PHA in 10% FCS-RPMI 1640 medium for 7 days (PHA-blasts) were incubated with differing numbers of cloned T cells in a 4-hour cytotoxicity assay. (•) NT4.2 (clone 12); (○) NT27 (clone 10).